Journal
APPLIED PHYSICS LETTERS
Volume 108, Issue 9, Pages -Publisher
AMER INST PHYSICS
DOI: 10.1063/1.4943265
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Funding
- Bundesministeriums fur Bildung und Forschung, BMBF [AZ. 031A318]
- Russian Foundation for Basic research [15-32-20324]
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We present a lifetime imaging technique that simultaneously records the fluorescence and phosphorescence lifetime images in confocal laser scanning systems. It is based on modulating a high-frequency pulsed laser synchronously with the pixel clock of the scanner, and recording the fluorescence and phosphorescence signals by multidimensional time-correlated single photon counting board. We demonstrate our technique on the recording of the fluorescence/phosphorescence lifetime images of human embryonic kidney cells at different environmental conditions. (C) 2016 AIP Publishing LLC.
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