Journal
BIOMEDICAL CHROMATOGRAPHY
Volume 29, Issue 12, Pages 1834-1841Publisher
WILEY
DOI: 10.1002/bmc.3504
Keywords
boronic acid affinity; HPLC-UV; MSPE; six-member covalent cyclic esters; SAL residual
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Funding
- National Natural Science Foundation of China [21275115, 20975080]
- Chinese Ministry of Education [212178]
- National High-Tech Research and Development Program of China (863 Program) [2012AA050103]
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Salbutamol (SAL) is the most widely used (2)-agonist drug for asthma and chronic obstructive pulmonary patients, but it is also often abused as feed additive. In recent years, the abuse of SAL has led to a large number of food safety incidents. Therefore, the monitoring of SAL residues in animal products is very important. A highly selective boronate affinity magnetic adsorbent was synthesized and developed for detection of trace levels of SAL residues in pig tissue samples. The obtained Fe3O4@SiO2@FPBA(4-formylphenylboronic acid) magnetic adsorbent showed good adsorption ability to catechol and SAL, and then it was successfully applied as special magnetic solid-phase phase extraction adsorbent coupled with high-performance liquid chromatography (HPLC) for simultaneous isolation and determination of cis-diol compounds. The binding capacity of catechol and SAL reached 96 and 50 mu mol/g, respectively. The method was successfully established for the detection of trace levels of SAL in pig tissue samples. The linear range extended from 0.32 to 800 mu g/kg (R-2 = 0.9994). The limit of detection of SAL was 0.19 mu g/kg. The recoveries were satisfactory (89.5-108.0%) at three spiked levels with RSD between 2.1 and 11.3%. These results indicated that the method has potential for enrichment and detection of trace levels of SAL residual in animal food products. Copyright (c) 2015 John Wiley & Sons, Ltd.
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