Deregulation of H19 is associated with cervical carcinoma

CACX is one of the most common cancer affecting women world-wide. Here, expression microarray analysis revealed 8 over-expressed transcribed pseudogenes (GBP1P1, HLA-DRB6, HLA-H, SLC6A10P, NAPSB, KRT16P2, PTTG3P and RNF126P1), down-regulated 7 lincRNAs (H19, MIR100HG, MEG3, DIO3OS, HOXA11-AS, CD27-AS1 and EPB41L4A-AS) and 6 snoRNAs (SNORD97, SNORD3A, SNORD3C, SNORD3D, SNORAI2 and SCARNA9) as DEncGs (log2 fold-change +/- 1.0) in CACX. Consequently, down-regulation of lincRNA MEG3 and over-ex- pression of pseudogenes, GBP1P1 and PTTG3P in the microarray analysis were found concordant with the realtime quantitative PCR results upon validation. Then, Ingenuity (R) Pathway analysis (IPA (R)) analysis with deregulated DEncGs identified functionally important gene, H19. Further, validation (n = 52) of expression confirmed frequent downregulation of H19 with significant association with its deletion (LOH) and promoter methylation (n = 128) in CACX. Moreover, clinicopathological analysis found Indian CACX patients (n = 26) with alterations of H19 by deletion or, promoter methylation with concomitant low expression have poor prognosis.