Journal
GENES AND NUTRITION
Volume 14, Issue -, Pages -Publisher
BMC
DOI: 10.1186/s12263-019-0644-8
Keywords
Allergy; FADS; ELOVL; Fish intake; DNA methylation; Pregnancy
Categories
Funding
- National Institutes of Health [R01AI091905, R01HL132321]
- NIH/NHLBI [R01 HL082925-01]
- European Respiratory Society
Ask authors/readers for more resources
BackgroundEvidence suggests that prenatal exposure to n-3 long-chain polyunsaturated fatty acids (LCPUFA) reduces the incidence of allergic disease in children. LCPUFAs are produced from dietary precursors catalyzed by desaturases and elongases encoded by the FADS1/2 and ELOVL5 genes. DNA methylation regulates gene activity and fatty acid supplementation could alter DNA methylation (DNA-M) at these genes. We investigated whether DNA-M and expression of the FADS1/2 and ELOVL5 genes were associated with allergy in children and gestational fish intake. We studied 170 participants from the Isle of Wight 3rd Generation Cohort, UK. Phenotype data and exposure was assessed by questionnaires. Genome-wide DNA-M in cord blood samples was quantified using the Illumina Infinium HumanMethylation450 and EPIC Beadchips. Five SNPs (single-nucleotide polymorphisms) in the FADS gene cluster and one SNP in ELOVL5 were genotyped in offspring. FADS gene expression in offspring cord blood was determined.ResultsGestational fish intake was significantly associated with increased methylation of cg12517394 (P=0.049), which positively correlated with FADS1 mRNA levels (P=0.021). ELOVL5 rs2397142 was significantly associated with eczema (P=0.011) and methylation at cg11748354 and cg24524396 (P<0.001 and P=0.036, respectively). Gestational fish intake was strongly associated with elevated DNA-M at cg11748354 and cg24524396 (P=0.029 and P=0.002, respectively) and reduced ELOVL5 mRNA expression (P=0.028).ConclusionThe association between induced FADS1/2 and ELOVL5 DNA-M and reduced gene expression due to gestational fish intake provide a mechanistic explanation of the previously observed association between maternal LCPUFA intake and allergy development in early childhood.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available