Journal
FOOD CHEMISTRY
Volume 283, Issue -, Pages 32-38Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2018.12.117
Keywords
Aptamer Aflatoxin B-1; Fluorescence resonance energy transfer; Nucleic acids probe; Homogeneous analysis; Rapid detection
Funding
- National Natural Science Foundation of China [21804095, 51773129]
- China Postdoctoral Science Foundation [2018M631079]
- Fundamental Research Funds for the Central Universities [2018SCU12048, 1083304121001]
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Aptamer probes provide an opportunity for achieving rapid and on-site detection of food contaminants. Herein, we proposed a general design strategy for aptamer probes enabling enzyme-free amplified, ultrafast and one-test tube homogeneous detection of aflatoxin B-1 (AFB(1)). The key feature of the aptamer probe is designed with dual-terminal proximity structures, allowing the binding of one molecule to light up two fluorophores, leading to enzyme-free amplification and a remarkable improvement of signal to background ratio and sensitivity for AFB1 detection. This aptamer probe could accommodate quick response to AFB1, and the detection process could be finished within 1 min, ranking one of the quickest assays for AFB1. AFB1 detection of broad bean paste and peanut oil conferred satisfactory recoveries ranging from 90.3% to 114.8%. Contributed to the generality and simplicity of the design strategy, this structure-switching probe could potentially act as a general platform of on-site detection for food safety.
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