Journal
FOOD CHEMISTRY
Volume 286, Issue -, Pages 129-135Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2019.01.215
Keywords
Hawthorn; Polysaccharide; Purification; Pectin oligosaccharides; Antiglycation activity
Funding
- National Natural Science Foundation of China [31301424/C200402]
- Foundation of Liaoning educational Committee [LQN201704]
- Open Project of Talent Small Highland in regard to Preservation and further processing of fruits and vegetables in Guangxi [2018GSXGD05]
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The crude polysaccharide was extracted from hawthorn (Crataegus pinnatifida Bunge. Var. major) and a salt-eluted polysaccharide (SPS-2) was fractionated by DEAE cellulose-52 chromatography. Monosaccharide composition and Fourier-transform infrared (FT-IR) analysis indicated that the SPS-2 contained 72.3% galacturonic acid and may be pectin. The results of antiglycation activity in vitro showed that the medium molecular weight pectin oligosaccharide (MM-POS) had the highest antiglycation activity among SPS-2 degradation products. Moreover, the MM-POS obtained by enzymatic degradation had stronger antiglycation activity than that by ultrasonic assisted enzymatic degradation. Composition analysis of MM-POS obtained from ultrasound-assisted enzymatic degradation showed that polygalacturonans content decreased from 85.1% to 61.9% but the arabinan and rhamnogalacturonans increased from 10.6% to 22.7% and from 3.5% to 13.2%, respectively, compared to the MM-POS obtained from enzymatic degradation. This study will provide a theoretical basis for the application of POSs in the food field, especially the exploitation of antiglycation agents.
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