Journal
FEBS LETTERS
Volume 593, Issue 18, Pages 2585-2595Publisher
WILEY
DOI: 10.1002/1873-3468.13526
Keywords
cerebral malaria; claudin-5; Erg; permeability; transcriptional regulation
Funding
- Medical Science and Technology Development Plan of Shandong Province [2017WS457]
- Shandong Taishan Scholarship
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Cerebral malaria (CM) is a severe complication with brain vascular hyperpermeability. Claudin-5 is the major component of tight junctions. To investigate the expression of claudin-5 in CM, we established a murine experimental cerebral malaria (ECM) model and an in vitro model by treating murine brain endothelial cells (bEnd3) with plasma from ECM mice. Expression of claudin-5 and the ETS transcription factor Erg was reduced in the brain endothelium of ECM mice. In bEnd3 cells exposed to ECM plasma, decreased expression of claudin-5 and Erg, and increased permeability were observed. Silencing of Erg significantly reduced Cldn5 expression. ChIP assays indicated that Erg binds to the -813 ETS motif of the murine Cldn5 gene promoter, and the binding is decreased by treatment with ECM plasma.
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