Journal
ENVIRONMENTAL RESEARCH
Volume 174, Issue -, Pages 46-53Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.envres.2019.04.018
Keywords
Zr-MOF; Organophosphate hydrolase; Bioconjugate; Enzyme activity; Pesticide; Biosensing
Funding
- Department of Biotechnology (DBT, India) [BT/PR18868/BCE/8/1370/2016]
- University Grant Commission (UGC, India)
- Science and Engineering Research Board (SERB), Department of Science and Technology (DST, Govt. of India, New Delhi) [PDF/2016/002182]
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The hexahistidine-tagged organophosphorus hydrolase (OPH6His) has been immobilized on a Zr-MOF, namely UiO-66-NH2. The resulting enzyme-MOF composite was used as a carrier to facilitate the hydrolysis of an organophosphate pesticide, i.e., methyl parathion in to p-nitrophenol (PNP). The formation of PNP took place in direct proportion to the added pesticide concentration. Coumarin1 (7-diethylamino-4-methylcoumarin) was then introduced in the reaction mixture as a reporter fluorescent molecule. As PNP acted to quench the fluorescence of coumarin1, it became possible to detect methyl parathion over a wide concentration range of 10-10(6) ng/mL with an achievable limit of quantification as 10 ng/mL. The immobilization of OPH6His on the surface of UiO-66-NH2 was found to endow an improvement in the enzymatic activity by about 37%. The OPH6(His)/UiO-66-NH2 conjugate was reusable for at least up to eight times and also found stable toward long-term storage (minimum 60 days). The potential practical utility of the above proposed sensing method has been demonstrated by employing it for an accurate analysis of pesticide-spiked food samples.
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