Salinity tolerance of quinoa (Chenopodium quinoa Willd) as assessed by chloroplast ultrastructure and photosynthetic performance

Quinoa has been widely used as a model crop for understanding salt-tolerance in halophyte plants. However, a salt tolerance mechanism regarding the photosynthetic activity is poorly evaluated. Here we report the effects of salt stress on photosynthesis of the halophyte Chenopodium quinoa cultivated under different NaCl concentrations (0, 100, and 300 mM). Our results revealed no apparent effect of moderate salinity (100 mM NaCl) on plant growth while, a reduction of plant biomass production was detected under 300 mM NaCl without any symptom of toxicity. No significant effect on the chloroplasts ultrastructure was observed under moderate salinity. Some morphological deformations of chloroplasts such as swelling of thylakoids, disruption of envelope, accumulation of starch grains and plastoglobuli were observed following prolonged exposure to severe stress. Under moderate salinity, no considerable change was detected on the rate of primary photochemistry (fluorescence O-J phase) and the reduction of the PQ pool (J-I phase), no apparent effects on the minimal (F-0), the maximal fluorescence (F-m) and the maximal photochemical efficiency (Fv/Fm) showing a high stability of PSII. The high PSII efficiency was also confirmed by the donor side intactness (constant Fk/Fj ratio) and stable antenna size. The high stability of PSII efficiency was also demonstrated by enhanced communication between antenna complex and PSII reaction centre and the stability of OEC complex (PsbQ and PsbO proteins). However, high salinity (300 mM NaCl) results in a swelling of thylakoids and disappearance of grana and in a decrease of Fm and (Fv/Fm) leading to the down-regulation of PSII activity. In addition, a significant increase in F0 occurred and could be associated to the presence of some Q(A)(-) in darkness in equilibrium with a partially reduced PQ pool. High salt treatment could be responsible for the thermal phase which induced an increase in chl a fluorescence (JIP rise with oxidized PQ pool).