Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 100, Issue 6, Pages 2535-2553Publisher
SPRINGER
DOI: 10.1007/s00253-016-7283-4
Keywords
LentiKats; Immobilization; Polyvinyl alcohol; Biocatalyst
Categories
Funding
- GRAIL [613667]
- European Commission under Seventh Framework Programme
- Slovak Research and Development Agency [DO7RP-0045-12, APVV-0656-11]
- Slovak Grant Agency for Science VEGA [1/0229/12]
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Biocatalyst immobilization is one of the techniques, which can improve whole cells or enzyme applications. This method, based on the fixation of the biocatalyst into or onto various materials, may increase robustness of the biocatalyst, allows its reuse, or improves the product yield. In recent decades, a number of immobilization techniques have been developed. They can be divided according to the used natural or synthetic material and principle of biocatalyst fixation in the particle. One option, based on the entrapment of cells or enzymes into a synthetic polyvinyl alcohol lens with original shape, is LentiKatsA (R) immobilization. This review describes the preparation principle of these particles and summarizes existing successful LentiKatsA (R) immobilizations. In addition, examples are compared with other immobilization techniques or free biocatalysts, pointing to the advantages and disadvantages of LentiKatsA (R).
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