Journal
EMBO REPORTS
Volume 20, Issue 8, Pages -Publisher
WILEY
DOI: 10.15252/embr.201847047
Keywords
angiogenesis; endothelial cell; Notch; VEGF; Wnt; zebrafish
Categories
Funding
- Wellcome Trust [210152/Z/18/Z] Funding Source: Wellcome Trust
- British Heart Foundation [PG/13/2/29902, PG/15/17/31332] Funding Source: Medline
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We identify a novel endothelial membrane behaviour in transgenic zebrafish. Cerebral blood vessels extrude large transient spherical structures that persist for an average of 23 min before regressing into the parent vessel. We term these structures kugeln, after the German for sphere. Kugeln are only observed arising from the cerebral vessels and are present as late as 28 days post fertilization. Kugeln do not communicate with the vessel lumen and can form in the absence of blood flow. They contain little or no cytoplasm, but the majority are highly positive for nitric oxide reactivity. Kugeln do not interact with brain lymphatic endothelial cells (BLECs) and can form in their absence, nor do they perform a scavenging role or interact with macrophages. Inhibition of actin polymerization, Myosin II, or Notch signalling reduces kugel formation, while inhibition of VEGF or Wnt dysregulation (either inhibition or activation) increases kugel formation. Kugeln represent a novel Notchdependent NO-containing endothelial organelle restricted to the cerebral vessels, of currently unknown function.
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