Journal
DEVELOPMENTAL CELL
Volume 49, Issue 6, Pages 936-+Publisher
CELL PRESS
DOI: 10.1016/j.devcel.2019.05.023
Keywords
-
Categories
Funding
- NIH [R01 GM 080484, R01 GM118712, T32 GM008061]
- Northwestern University
- JSPS Overseas postdoctoral fellowship from the Japanese Society for the Promotion of Science
Ask authors/readers for more resources
Loss of nuclear pore complex (NPC) proteins, transcription factors (TFs), histone modification enzymes, Mediator, and factors involved in mRNA export disrupts the physical interaction of chromosomal sites with NPCs. Conditional inactivation and ectopic tethering experiments support a direct role for the TFs Gcn4 and Nup2 in mediating interaction with the NPC but suggest an indirect role for factors involved in mRNA export or transcription. A conserved positioning domain within Gcn4 controls interaction with the NPC and inter-chromosomal clustering and promotes transcription of target genes. Such a function may be quite common; a comprehensive screen reveals that tethering of most yeast TFs is sufficient to promote targeting to the NPC. While some TFs require Nup100, others do not, suggesting two distinct targeting mechanisms. These results highlight an important and under appreciated function of TFs in controlling the spatial organization of the yeast genome through interaction with the NPC.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available