Journal
CLINICAL IMMUNOLOGY
Volume 203, Issue -, Pages 63-71Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.clim.2019.04.006
Keywords
gamma delta T cells; Neuroblastoma; DNAM-1; Cytolytic activity
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Funding
- National Natural Science Foundation of China [81772668]
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Neuroblastoma is one of the children's malignant tumors with poor prognosis, as well as high recurrence and metastasis rates after surgical removal and chemotherapy. gamma delta T-cell based immunotherapy receives increasing attention thanks to the strong cytolytic activity to tumor cells. Our previous data revealed a significant increase in circulating gamma delta T-cell frequency in NB patients. In the present study, we found that beside a reduction of IFN-gamma in serum of NB patients, DNAM-1 expression decreased in both circulating and PAM-expanded NB gamma delta T cells. Upon PAM stimulation, NB gamma delta T cells showed a reduced level of cell proliferation. In addition, the cytolytic activity of NB gamma delta T cells to NB cell lines was proved to be attenuated in a co-culture system. The fact that DNAM-1 neutralizing antibody abolished the tumor cell killing accentuates the indispensable role of DNAM-1 molecule in gamma delta T-cell cytolytic function.
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