Journal
CHINESE CHEMICAL LETTERS
Volume 31, Issue 2, Pages 447-450Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cclet.2019.07.046
Keywords
Two-photon; Lysosome-targeting; Polarity; Real-time; Autophagy
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Funding
- National Natural Science Foundation of China [21778001, 21672001]
- Open Fund for Discipline Construction in Institute of Physical Science and Information Technology of Anhui University
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A novel donor-acceptor (D-A) type of two-photon (TP) fluorescent probe, i.e. Lyso-OSC, based on the lysosome-targeting morpholine group was developed. The polarity sensing coumarin group was functionalized as the acceptor and the 1-vinyl-4-methoxybenzene group was engineered as the donor. The fluorescence intensity and emission maximum wavelength of Lyso-OSC are highly sensitive to the polarity changes of solvent. The two-photon absorption cross-section and tissue penetration depth are up to 254 GM and 150 mu m, respectively. The strong fluorescence, high sensitivity to polarity, low cytotoxicity, and accurate lysosome-targeting ability entail Lyso-OSC the excellent performance in detecting the polarity changes of cellular environment. To this end, a bright, real-time imaging autophagy of living cells has been achieved. (C) 2019 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.
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