Journal
CHEMISTRY-A EUROPEAN JOURNAL
Volume 25, Issue 56, Pages 12946-12956Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201902263
Keywords
glycoconjugate vaccines; NMR spectroscopy; polysaccharides; squaric acid; Vibrio cholerae
Categories
Funding
- Intramural Research Program of the National Institutes of Health (NIH)
- National Institute of Diabetes and Digestive and Kidney Diseases [DK059701]
- Extramural Research Program of the National Institute of Allergy and Infectious Diseases [R01 AI106878]
Ask authors/readers for more resources
The lipopolysaccharide (LPS) of Vibrio cholerae O139, strain CIRS245, was isolated conventionally, and the lipid A was removed by mild acid hydrolysis (0.1 m NaOAc buffer containing 1 % SDS, pH 4.2, 95 degrees C, 8 h). The crude product was a complex mixture consisting mainly of constituent fragments of the O-specific polysaccharide-core (OSPc). The OSPc was only a minor component in the mixture. Two-stage purification of the crude OSPc by HPLC gave pure OSPc fragment of the LPS, as shown by NMR spectroscopy, analytical HPLC and ESI-MS. This material is the purest OSPc fragment of the LPS from Vibrio cholerae O139 reported to date. The purified OSPc was readily converted to the corresponding methyl squarate derivative and the latter was conjugated to BSA. The conjugate, when examined by ELISA, showed immunoreactivity with sera from patients in Bangladesh recovering from cholera caused by V. cholerae O139, but not O1.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available