Journal
CHEMISTRY & BIODIVERSITY
Volume 16, Issue 8, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbdv.201900299
Keywords
huperzine B; biotransformation; fungal endophyte; Bjerkandera adusta; Huperzia serrata; biological activity
Funding
- Natural Science Foundation of Zhejiang Province [LY17B020011]
- Natural Science Foundation of China [21402174]
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The biotransformation of huperzine B (hupB), one of the characteristic bioactive constituents of the medicinal plant Huperzia serrata, by a fungal endophyte of the host plant was studied. One new compound, 8 alpha,15 alpha-epoxyhuperzine B (1), along with two known oxygenated hupB analogs, 16-hydroxyhuperzine B (2) and carinatumin B (3), was isolated and identified. The structures of all the isolates were deduced by spectroscopic methods including NMR, MS, IR, and UV spectra. The known compounds 2 and 3 were obtained from a microbial source for the first time. To the best of our knowledge, it is the first report on the microbial transformation of hupB and would facilitate further structural modification of hupB by chemo-enzymatic method. In the LPS-induced neuro-inflammation injury assay, 8 alpha,15 alpha-epoxyhuperzine B (1) exhibited moderate neuroprotective activity by increasing the viability of U251 cell lines with an EC50 of 40.1 nm.
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