Journal
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
Volume 35, Issue 4, Pages 581-585Publisher
HIGHER EDUCATION PRESS
DOI: 10.1007/s40242-019-9085-6
Keywords
DNA-silver nanocluster; Ratiometric; Binary probe; Human papillomavirus
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Funding
- National Natural Science Foundation of China [21675047, 21735002, 21521063]
- Key Point Research and Invention Program of Hunan Province, China [2017DK2011]
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Herein, we described a ratiometric strategy based on chameleon DNA-silver nano-clusters(DNA-AgNCs) fluorescent binary probes. The strategy was applied to detect high-risk human papillomavirus(HPV) DNA sequences, HPV-16. First, DNA-AgNCs were synthesized by a simple reduction method. The obtained nanoprobes showed typical yellow and red fluorescence of AgNCs. Upon the addition of HPV-16 DNA, the yellow fluorescence of AgNCs was reduced greatly, whereas the red fluorescence of AgNCs was increased. The concentration of HPV-16 DNA in the samples was characterized by the ratio of fluorescence intensity at 570 and 630 nm. The ratiometric nanoprobes showed good selectivity for HPV-16 DNA, and the detection limit was 2 nmol/L. In addition, the practical applicability of this strategy was demonstrated by analysing the HPV-16 DNA in human serum, illustrating its potential promise for clinical diagnosis.
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