Journal
CHEMBIOCHEM
Volume 21, Issue 4, Pages 451-460Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201900291
Keywords
EPR spectroscopy; in-cell spectroscopy; SDSL; spin labels; structural biology
Funding
- Agence Nationale de la Recherche [ANR15CE29-0022-02, ANR-18-CE11-0007-01]
- Conseil Regional of Region Sud (APEX-2015)
- Agence Nationale de la Recherche (ANR) [ANR-18-CE11-0007] Funding Source: Agence Nationale de la Recherche (ANR)
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Exploring the structure and dynamics of biomolecules in the context of their intracellular environment has become the ultimate challenge for structural biology. As the cellular environment is barely reproducible in vitro, investigation of biomolecules directly inside cells has attracted a growing interest. Among magnetic resonance approaches, site-directed spin labeling (SDSL) coupled to electron paramagnetic resonance (EPR) spectroscopy provides competitive and advantageous features to capture protein structure and dynamics inside cells. To date, several in-cell EPR approaches have been successfully applied to both bacterial and eukaryotic cells. In this review, the major advances of in-cell EPR spectroscopy are summarized, as well as the challenges this approach still poses.
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