Journal
CARBOHYDRATE POLYMERS
Volume 216, Issue -, Pages 204-212Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2019.04.017
Keywords
Antrodia cinnamomea; Sulfated polysaccharides; Sodium thiosulfate; Anticancer activity
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Funding
- Ministry of Science and Technology (Taiwan) [MOST107-2636-B-010-003, MOST105-2320-B-077-002MY3]
- Development and Construction Plan of the School of Medicine, National Yang-Ming University [107F-M01-07M32]
- Ministry of Health and Welfare [MOHW107NRICM-B-325-000202]
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Sulfated polysaccharides (SPSs) are polysaccharides (PSs) with high sulfate functionalization and possess bioactivities. This study aimed to increase the sulfate content of SPSs in Antrodia cinnamomea through sulfate feeding. Feeding A. cinnamomea with sodium thiosulfate was found to increase yields of PSs and SPSs in A. cinnamomea. The SPSs thus obtained (ST-SPS) were further isolated, showing enhanced sulfate content of 2.5 mmol/g. Sodium thiosulfate induced changes in molecular weight from 320 kDa to 1342 kDa, and area percentage of low-molecular-weight ST-SPS (< 20 kDa) was decreased. Functional studies revealed that sodium thiosulfate increased the ST-SPS anticancer efficacy in cancer cells via inhibition of EGFR/AKT signaling. Moreover, the ST-SPS enhanced synergistically cisplatin-, gefitinib- and 5 FU-induced cytotoxic effects in lung cancer H1975 cells and colon cancer CT26 cells. This study is the first to demonstrate that sodium thiosulfate induced changes in properties of A. cinnamomea with the anticancer mechanisms of ST-SPS.
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