Journal
CANCER LETTERS
Volume 452, Issue -, Pages 1-13Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2019.03.017
Keywords
TRIP4; Cervical cancer; Radioresistance; AKT/MAPK; Telomerase
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Funding
- National Natural Science Foundation of China [81473452, 81703904, 81472178, 81272195]
- Education Department of Liaoning Province, China (the Program for Distinguished Professor of Liaoning Province)
- Education Department of Liaoning Province, China (the Scientific Research Project) [L2015142]
- Education Department of Liaoning Province, China (Construction Project of Traditional Chinese Medicine of Liaoning Province)
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Thyroid hormone receptor interactor 4 (TRIP4), a subunit of the tetrameric nuclear activating signal co-integrator 1 (ASC-1) complex, exerts pro-tumorigenic effects. The role for TRIP4 in the regulation of cervical cancer growth and radiation resistance is presently unknown. In this study, TRIP4 was found to be highly expressed in cervical cancer cells and tumor tissues. Knockdown of TRIP4 significantly suppressed cervical cancer cell proliferation and epithelial-mesenchymal transition (EMT), accompanied by inactivation of PI3K/AKT and MAPK/ERK signaling. TRIP4 was also found to target hTERT signaling by regulating its binding to the hTERT promoter. Moreover, the knockdown of TRIP4 increased cell sensitivity to radiation, concomitant with downregulation of Rad51 and p-H2AX. We also demonstrated in an in vivo study that the knockdown of TRIP4 effectively suppressed cervical cancer growth and progression in a xenograft tumor model, and these effects were concomitant with the downregulation of p-AKT, p-ERK, p-MEK1/2, MMP-9 and hTERT expression. Immunohistochemical analysis of tumor tissue microarrays showed that TRIP4 overexpression predicted poor prognosis in patients with cervical cancer. Collectively, these results show that TRIP4 plays an essential role in cervical cancer growth and survival.
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