Journal
BIOMATERIALS
Volume 56, Issue -, Pages 68-77Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2015.03.053
Keywords
Silk fibroin; Vascularization; Cell survival; Cell tracking; Regenerative medicine
Funding
- National Basic Research Program of China (973 Program) [2012CB933604]
- Ph.D Innovation Fund Projects from Shanghai Jiao Tong University School of Medicine [BXJ201331]
- National Science Fund for Distinguished Young Scholars of China [81225006]
- National Natural Science Foundation of China [81430012, 81170939]
- National Institutes of Health [P41 EB002520]
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Despite the promise for stem cell-based tissue engineering for regenerative therapy, slow and insufficient vascularization of large tissue constructs negatively impacts the survival and function of these transplanted cells. A combination of channeled porous silk scaffolds and prevascularization with endothelial cells was investigated to test the ability of this tissue engineering strategy to support rapid and extensive vascularization process. We report that hollow channels promote in vitro prevascularization by facilitating endothelial cell growth, VEGF secretion, and capillary-like tube formation. When implanted in vivo, the pre-established vascular networks in the hollow channel scaffolds anastomose with host vessels and exhibit accelerated vascular infiltration throughout the whole tissue construct, which provides timely and sufficient nutrients to ensure the survival of the transplanted stem cells. This tissue engineering strategy can promote the effective application of stem cell-based regeneration to improve future clinical applications. (C) 2015 Elsevier Ltd. All rights reserved.
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