Journal
BIOMATERIALS
Volume 36, Issue -, Pages 90-97Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2014.09.014
Keywords
Human mesenchymal stem cell (hMSC); Stem cell engineering; Stem cell homing; Tumor-targeted delivery; Cancer diagnosis
Funding
- National Research Foundation of Korea (NRF) grant - Korea government (MSIP) [2011-0028726]
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To improve the gadolinium (Gd) internalization efficiency in stem cells, gadolinium-chelate nanoparticles were prepared from a pullulan derivative (pullulan-deoxycholic acid (DOCA)-diethylene triamine pentaacetic (DTPA)-Gd conjugate; PDDG) and then the PDDG was entrapped into human mesenchymal stem cells (hMSCs) by the photochemical-internalization (PCI) method for cancer diagnosis via the cancer homing property of hMSCs. The internalization efficiency of Gd in hMSCs was significantly increased to 98 +/- 4 pg Gd/cell from 32 +/- 2 pg Gd/cell via the PCI method. Moreover, the Gd-entrapped hMSCs revealed a low exocytosis ratio of gadolinium-chelate nanoparticles during cell division in vitro and a high cellular labeling efficiency for at least 21 days in vivo. The cancer-targeting and diagnosis effect of the Gd-entrapped hMSCs were confirmed in a small CT26 tumor-bearing mice model. The stem cells detected an early tumor (similar to 3 mm(3)) within 2 h using 4.7-T MR and optical imaging. The results demonstrated that the PCI-mediated internalization of Gd-incorporated nanoparticles into hMSCs is a promising protocol for efficient cell labeling and tracking. (C) 2014 Elsevier Ltd. All rights reserved.
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