4.3 Article

Complete Genome Sequence of Lactic Acid Bacterium Pediococcus acidilactici Strain ATCC 8042, an Autolytic Anti-bacterial Peptidoglycan Hydrolase Producer

Journal

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
Volume 24, Issue 3, Pages 483-487

Publisher

KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
DOI: 10.1007/s12257-019-0037-2

Keywords

Pediococcus acidilactici ATCC 8042; complete genome sequence; peptidoglycan hydrolase; antimicrobial activity

Funding

  1. Bio & Medical Technology Development Program of the National Research Foundation (NRF) - Ministry of Science and ICT (MSIT) [NRF-2018M3A9H3020459]
  2. Next-Generation BioGreen 21 Program - Rural Development Administration [PJ01323601]
  3. Creative-Pioneering Researchers Program through Seoul National University (SNU)

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Pediococcus acidilactici is a probiotic bacterium that is industrially utilized in the food industry and antibiotics development. Here, we determine the complete nucleotide sequence of the genome of Pediococcus acidilactici ATCC 8042. The genome was sequenced by the PacBio RSII to generate a single contig consisting of circular chromosome sequence. Illumina MiniSeq sequencing platform and Sanger sequencing method were additionally utilized to correct errors resulting from the long-read sequencing platform. The sequence consists of 2,009,598 bp with a G + C content of 42.1% and contains 1,865 protein-coding sequences. Based on the sequence information, we could confirm and predict the presence of four peptidoglycan hydrolases by HyPe software. This work, therefore, provides the complete genomic information of P. acidilactici ATCC 8042 with a profitable potential of genome-scale comprehension of anti-pathogenic activity, which can be applied in nutraceutical and pharmaceutical biotechnology field.

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