Journal
BIORESOURCE TECHNOLOGY
Volume 284, Issue -, Pages 381-390Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2019.03.148
Keywords
Wet Nannochloropsis biomass; Lipids; Enzyme assisted three phase partitioning; Extraction; Lipid class
Funding
- 13th Five-year National Innovation and Development of Marine Economy Demonstration Project [FZHJ04]
- Projects of Department of Science and Technology of Fuzhou City of Fujian Province of China [2016-G-73, 2017-G-57]
- Key Projects of Department of Science and Technology of Fujian Province of China, China [2019N0011]
- Key Laboratory of Feed Biotechnology (The Ministry of Agriculture of the People's Republic of China)
Ask authors/readers for more resources
A green and efficient enzyme assisted three phase partitioning (EA-TPP) process was firstly developed to extract microalgal lipids using wet Nannochloropsis sp. biomass. In the pretreatment of microalgal biomass by four hydrolytic enzymes, TPP obtained a higher TFAs lipid extraction efficiency by cellulase compared with the resting enzymes. After optimization by EA-TPP of the wet disrupted Nannochloropsis biomass (3 g), the maximum TFAs extraction yield (90.40%) was attained at 20% ammonium sulphate, 6-7 pH, 1:2 slurry/tert-butanol ratio and 70 degrees C for 2 h incubation time and two extraction cycles. Moreover, results also revealed that the lipidic species compositions of Nannochloropsis sp. biomass were greatly related with the EA-TPP parameters. In the laboratory scale for wet disrupted microalgae biomass, EA-TPP process achieved 88.70% TFAs extraction yield under the optimized conditions. In all, EA-TPP process could be a promising approach to extract microalgae lipids for food application using wet microalgae biomass.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available