Journal
BIOMASS & BIOENERGY
Volume 126, Issue -, Pages 190-198Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biombioe.2019.05.011
Keywords
Hemicellulosic hydrolysate; Xylose; Wild-type strain; Clostridium saccharoperbutylacetonicum; Butanol tolerance
Funding
- Brazilian National Council for Scientific and Technological Development (CNPq) [141465/2015-8, 400803/2013-5, 165075/2017-1]
- Sao Paulo Research Foundation (FAPESP) [2015/20630-4]
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Robust strains are essential towards success of n-butanol production from lignocellulosic feedstock. To find a suitable strain to convert a non-detoxified hemicellulosic hydrolysate of sugarcane bagasse, we first assessed the performance of four wild-type butanol-producing Clostridium strains (C. acetobutylicum DSM 6228, C. beijerinckii DSM 6422, C. saccharobutylicum DSM 13864, and C. saccharoperbutylacetonicum DSM 14923) in batch fermentations containing either xylose or glucose at 30 g L-1 as sole carbon sources. C. saccharoperbutylacetonicum was selected after achieving butanol yields as high as 0.31 g g(-1) on glucose and 0.25 g g(-1) on xylose. In a 48-h fermentation containing a mixture of sugars (93% xylose and 7% glucose) that mimicked the hydrolysate, C. saccharoperbutylacetonicum delivered the highest butanol concentration (14.5 g L-1) when the initial sugar concentration was 50 g L-1. Moreover, the selected strain achieved the highest butanol yield (0.29 g g(-1)) on xylose-rich media reported so far. Meanwhile, C. saccharoperbutylacetonicum produced 5.8 g butanol L-1 (0.22 g g(-1) butanol yield) when fermenting a non-detoxified sugarcane bagasse hemicellulosic hydrolysate enriched with xylose (30 g total sugars L-1). Although sugars were not exhausted (4.7 g residual sugars L-1) even after 72 h because of the presence of lignocellulose-derived microbial inhibitors, these results show that C. saccharoperbutylacetonicum is a robust wild-type strain. This microorganism with high butanol tolerance and yield on xylose can, therefore, serve as the basis for the development of improved biocatalysts for production of butanol from non-detoxified sugarcane bagasse hemicellulosic hydrolysate.
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