Journal
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
Volume 1867, Issue 6, Pages 595-603Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbapap.2019.04.001
Keywords
Cytochrome c(GJ); Methylotrophy; Verrucomicrobia; Acidophile; Methanol dehydrogenase; Cytochrome; Electron transfer; Heme; Metalloprotein
Categories
Funding
- ERC AG Ecomom [339880]
- ERCAG VOLCANO [669371]
- Netherlands Organization for Scientific Research [NWO] [824.15.011]
- National Science Foundation (USA) [CHE-0820965]
- Deutsche Forschungsgemeinschaft (DFG) [392552271]
- CIPSM
- European Research Council (ERC) [339880, 669371] Funding Source: European Research Council (ERC)
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Methanotrophs play a prominent role in the global carbon cycle, by oxidizing the potent greenhouse gas methane to CO2. Methane is first converted into methanol by methane monooxygenase. This methanol is subsequently oxidized by either a calcium-dependent MxaF-type or a lanthanide-dependent XoxF-type methanol dehydrogenase (MDH). Electrons from methanol oxidation are shuttled to a cytochrome redox partner, termed cytochrome cc. Here, the cytochrome c(L) homolog from the thermoacidophilic methanotroph Methylacidiphilum fumariolicum SolV was characterized. SolV cytochrome c(GJ) is a fusion of a XoxG cytochrome and a periplasmic binding protein XoxJ. Here we show that XoxGJ functions as the direct electron acceptor of its corresponding XoxF-type MDH and can sustain methanol turnover, when a secondary cytochrome is present as final electron acceptor. SolV cytochrome C-GJ (XoxGJ) further displays a unique, red-shifted absorbance spectrum, with a Soret and Q bands at 440, 553 and 595 nm in the reduced state, respectively. VTVH-MCD spectroscopy revealed the presence of a low spin iron heme and the data further shows that the heme group exhibits minimal ruffling. The midpoint potential E-m,E-pH7 of + 240 mV is similar to other cytochrome c(L) type proteins but remarkably, the midpoint potential of cytochrome c(GJ) was not influenced by lowering the pH. Cytochrome c(GJ) represents the first example of a cytochrome from a strictly lanthanide-dependent methylotrophic microorganism.
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