Journal
BIOCHEMISTRY
Volume 58, Issue 30, Pages 3243-3250Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.biochem.9b00404
Keywords
-
Categories
Funding
- Australian Research Council [DP170100162, FL170100019]
- Laureate Fellowship [FL170100019]
- Australian Research Council [FL170100019] Funding Source: Australian Research Council
Ask authors/readers for more resources
Pseudocontact shifts (PCSs) generated by paramagnetic metal ions contribute highly informative long-range structure restraints that can be measured in solution and are ideally suited to guide structure prediction algorithms in determining global protein folds. We recently demonstrated that PCSs, which are relatively small but of high quality, can be generated by a double-histidine (dHis) motif in an alpha-helix, which provides a well-defined binding site for a single Co2+ ion. Here we show that PCSs of backbone amide protons generated by dHis-Co2+ motifs positioned in four different alpha-helices of a protein deliver excellent restraints to determine the three-dimensional (3D) structure of a protein in a way akin to the global positioning system (GPS). We demonstrate the approach with GPS-Rosetta calculations of the 3D structure of the C-terminal domain of the chaperone ERp29 (ERp29-C). Despite the relatively small size of the PCSs generated by the dHis-Co2+ motifs, the structure calculations converged readily. Generating PCSs by the dHis-Co2+ motif thus presents an excellent alternative to the use of lanthanide tags.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available