Journal
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
Volume 676, Issue -, Pages -Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.abb.2019.07.020
Keywords
Experimental assessment of protein disorder; Degree of compactness; Secondary and tertiary structure content; Ensemble models; In-vivo assessment of disorder
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Funding
- French Ministry of National Education, Research and Technology
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In this review, we detail the most common experimental approaches to assess and characterize protein intrinsic structural disorder, with the notable exception of NMR and EPR spectroscopy, two ideally suited approaches that will be described in depth in two other reviews within this special issue. We discuss the advantages, the limitations, as well as the caveats of the various methods. We also describe less common and more demanding approaches that enable achieving further insights into the conformational properties of IDPs. Finally, we present recent developments that have enabled assessment of structural disorder in living cells, and discuss the currently available methods to model IDPs as conformational ensembles.
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