Journal
BIOMATERIALS
Volume 41, Issue -, Pages 15-25Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2014.11.019
Keywords
Adipose-derived stem cells; Microgravity bioreactor; Sternness property; Therapeutic potential
Funding
- National Program on Key Basic Research Project of China [2011CB964701]
- National Natural Science Foundation of China [30800237]
- Foundation for Science and Technology Research Project of Chongqing [CSTC2012GGB1003]
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Adipose-derived stem cells (ADSCs) represent a valuable source of stem cells for regenerative medicine, but the loss of their sternness during in vitro expansion remains a major roadblock. We employed a microgravity bioreactor (MB) to develop a method for biomaterial-free-mediated spheroid formation to maintain the sternness properties of ADSCs. ADSCs spontaneously formed three-dimensional spheroids in the MB. Compared with monolayer culture, the expression levels of E-cadherin and pluripotent markers were significantly upregulated in ADSC spheroids. Spheroid-derived ADSCs exhibited increased proliferative ability and colony-forming efficiency. By culturing the spheroid-derived ADSCs in an appropriate induction medium, we found that the multipotency differentiation capacities of ADSCs were significantly improved by spheroid culture in the MB. Furthermore, when ADSCs were administered to mice with carbon tetrachloride-induced acute liver failure, spheroid-derived ADSCs showed more effective potentials to rescue liver failure than ADSCs derived from constant monolayer culture. Our results suggest that spheroid formation of ADSCs in an MB enhances their sternness properties and increases their therapeutic potential. Therefore, spheroid culture in an MB can be an efficient method to maintain stemness properties, without the involvement of any biomaterials for clinical applications of in vitro cultured ADSCs. (C) 2014 Elsevier Ltd. All rights reserved.
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