Journal
APPLIED OPTICS
Volume 58, Issue 18, Pages 4892-4897Publisher
OPTICAL SOC AMER
DOI: 10.1364/AO.58.004892
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Funding
- National Key Research and Development Program of China [2017YFC0110303]
- National Basic Research Program of China (973Program) [2015CB352003]
- National Natural Science Foundation of China (NSFC) [61427818, 61735017, 61827825]
- Natural Science Foundation of Zhejiang Province [LR16F050001]
- Fundamental Research Funds for the Central Universities [2018FZA5005]
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An axial-resolution-enhanced two-photon laser scanning microscopy system is presented in this paper. In the proposed method, we use a spatial light modulator (SLM) for the phase modulation of the excitation light. The axially split point spread function (PSF) is generated by loading a 0 - pi pattern on the SLM. The final quality-enhanced images are acquired by subtracting the two consecutive images acquired by the original PSF and the split PSF. Because of the fluorescence differential processing, the axial elongation of the particles images is suppressed, and the axial resolution is enhanced accordingly. With the axial resolution enhanced, the overlap between layer images is also reduced, which decreases the background noise of the images and enhances the contrast and image quality of the acquired fluorescence images. The capability of axial resolution and contrast enhancement is successfully demonstrated by both theoretical calculation and experimental results. (C) 2019 Optical Society of America
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