Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 85, Issue 19, Pages -Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.01548-19
Keywords
cocultivation; Euglena gracilis; IAA; paramylon; Vibrio natriegens
Categories
Funding
- Marine Biotechnology Program of the Korea Institute of Marine Science and Technology Program - Ministry of Oceans and Fisheries [20170488]
- National Research Foundation of Korea - Korean government (Ministry of Science, ICT and Future Planning [MSIP]) [2016R1D1A1B03932773]
- National Research Foundation of Korea - Korean government (NRF-2018 Global Ph.D. Fellowship program)
- National Research Foundation of Korea [2016R1D1A1B03932773] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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We investigated the putative effects on the growth and paramylon production of Euglena gracilis of cocultivation with Vibrio natriegens. E. gracilis heterotrophically cocultivated with V. natriegens displayed significant increases in biomass productivity and paramylon content. In addition, the effects of the bacterial inoculum density and the timing of inoculation on the growth of E. gracilis were examined, to determine the optimal conditions for cocultivation. With the optimal deployment of V. natriegens, biomass productivity and paramylon content were increased by more than 20% and 35%, respectively, compared to those in axenic E. gracilis cultures. Interestingly, indole-3-acetic acid biosynthesized by V. natriegens was responsible for these enhancements of E. gracilis. The morphology of cocultured E. gracilis cells was assessed. Paramylon granules extracted from the cocultivation were significantly larger than those from axenic culture. Our study showed that screening for appropriate bacteria and subsequent cocultivation with E. gracilis represented an effective way to enhance biomass and metabolite production. IMPORTANCE Euglena gracilis has attracted special interest due to its ability to excessively accumulate paramylon. Paramylon is a linear beta-1,3-glucan polysaccharide that is the principal polymer for energy storage in E. gracilis. The polysaccharide features high bioactive functionality in the immune system. This study explored a new method to enhance the production of paramylon by E. gracilis, through cocultivation with the indole-3-acetic acid-producing bacterium Vibrio natriegens. The enhanced production was achieved indirectly with the phytohormone-producing bacteria, instead of direct application of the hormone. The knowledge obtained in this study furthers the understanding of the effects of V. natriegens on the growth and physiology of E. gracilis.
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