Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 58, Issue 35, Pages 12195-12199Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201907605
Keywords
circulating tumor cells; in vitro biodetection; lanthanide; fluorescent probes; time-resolved spectroscopy
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Funding
- Strategic Priority Research Program of the CAS [XDB20000000]
- NSFC [81572944, 21771185, 21804134, 51672272]
- CAS/SAFEA International Partnership Program for Creative Research Teams
- Natural Science Foundation of Fujian Province [2017I0018, 2019J05157, 2017J01030]
- China Postdoctoral Science Foundation [2017LH017, 2018M630735]
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The detection of circulating tumor cells (CTCs) is crucial to early cancer diagnosis and the evaluation of cancer metastasis. However, it remains challenging due to the scarcity of CTCs in the blood. Herein, we report an ultrasensitive platform for the direct detection of CTCs using luminescent lanthanide nanoprobes. These were designed to recognize the epithelial cell adhesion molecules on cancer cells, allowing signal amplification through dissolution-enhanced time-resolved photoluminescence (TRPL) and the elimination of short-lived autofluorescence interference. This enabled the direct detection of blood breast-cancer cells with a limit of detection down to 1 cell/well of a 96-well plate. Moreover, blood CTCs (>= 10 cells mL(-1)) can be detected in cancer patients with a detection rate of 93.9 % (14/15 patients). We envision that this ultrasensitive detection platform with excellent practicality may provide an effective strategy for early cancer diagnosis and prognosis evaluation.
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