Journal
ANALYTICAL CHEMISTRY
Volume 91, Issue 13, Pages 8524-8531Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.9b01779
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Funding
- National Natural Science Foundation of China [21572093, 21778028]
- Natural Science Foundation of Gansu Province [18JR4RA003]
- Ministry of Science and Technology National Key RD project [SQ2018YFE020524]
- 111 project
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Finding specific small molecule probes of a biological target is extremely desired but remains a big challenge. We reported herein a highly selective fluorescent probe derivatized from the nile blue fluorophore, NBL-SS, for thioredoxin (Trx), a ubiquitous redox-regulating protein essentially involved in cell growth, differentiation, and death. NBL-SS displayed multiple favorable properties, such as red emission, fast response, and high fluorescence signal, which enabled the probe to readily image Trx functions in live cells and in vivo. The fluorophore-dependent selectivity indicates that manipulation of weak interactions between probes and their target biomacromolecules could further improve the probes' specificity. In addition, our discovery, i.e., the preference reduction of simple disulfide bonds by Trx over glutathione, also advances the development of disulfide cleavage-based probes, prodrugs, and theranostic agents.
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