Monitoring the Changes of pH in Lysosomes during Autophagy and Apoptosis by Plasmon Enhanced Raman Imaging

Lysosomes are acidic organelles that not only participate in intracellular degradation but also relate to various cellular functions. Abnormal pH in lysosomes would lead to lysosomal dysfunction, which may further result in many diseases. In this work, we statistically analyze the pH change in the lysosomes of HeLa cells model by using surface enhanced Raman scattering (SERS) imaging technique. We prepared a plasmon Raman pH probe and localized the pH probe to lysosomes via an incubation-depletion method. The pH profiles within lysosomes during the process of cellular autophagy and apoptosis were monitored in situ by SERS imaging. The pH in lysosomes decreased slightly during the process of autophagy, while the pH in lysosomes increased during apoptosis. The phenomenon, in general, is consistent with our current biological knowledge. However, we did not observe significant variation of pH between different individual cells. This information might provide an in depth understanding about the relationship of lysosomal pH with fundamental cellular functions and mechanism of diseases.