Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 411, Issue 24, Pages 6329-6341Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-019-01931-x
Keywords
Tara; Gallic acid derivatives; Gallotannins; Hydrophilic interaction chromatography (HILIC); Reversed-phase liquid chromatography (RP-LC); Ion mobility (IM) spectrometry high-resolution mass spectrometry (HR-MS)
Funding
- Sasol
- National Research Foundation (NRF) [98897]
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Reversed-phase liquid chromatography (RP-LC) and hydrophilic interaction chromatography (HILIC) methods hyphenated to diode array detection and ion mobility (IM) high-resolution mass spectrometry (HR-MS) were used for the analysis of gallic acid derivatives and gallotannins in a commercial tara extract. UV spectra combined with low and high-collision energy mass spectral data and known RP-LC elution orders allowed the identification of 45 isomeric gallic acid derivatives and gallotannins. The synergy between IM and UV data was found to provide a simple means to determine the number of depsidic bonds and thus to distinguish between positional isomers. IM also facilitated the assignment of individual isomeric species between HILIC and RP-LC separations. For the hydrolysable tannins present in tara, RP-LC provided superior resolution and specificity compared to HILIC. The results reported in this paper highlight the utility of IM in combination with optimised complementary chromatographic separations and HR-MS for the detailed qualitative analysis of hydrolysable tannins in complex mixtures of these compounds.
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