Journal
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
Volume 82, Issue 3, Pages -Publisher
WILEY
DOI: 10.1111/aji.13155
Keywords
acrosome reaction; leukocytospermia; oxidative stress; Quercetin; sperm function
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Funding
- Guangdong Natural Science Foundation Funding Source: Medline
- National Natural Science Foundation of China [2014A030313785, 2014A030313652, 81300530, 81401258] Funding Source: Medline
- Natural Science Foundation of Guangdong Province [2014A030313785, 2014A030313652] Funding Source: Medline
- Medical Scientific Research Foundation of Guangdong [A2015408, A2015122] Funding Source: Medline
- Shenzhen Foundation of Science and Technology [JCYJ20150330102401083] Funding Source: Medline
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Problem Quercetin has been shown to display intensive antioxidant activity against ROS-mediated damage in chilled semen, and the effects and molecular mechanisms of Quercetin on sperm function in the infertile patients with leukocytospermia remain largely unknown. Methods Semen samples were collected from the infertile men with leukocytospermia (n = 56) and fertile men (n = 44). Computer-assisted semen analysis (CASA) was used to determine sperm motility before and after Quercetin incubation (10 mu mol/L). Changes in H2O2, sperm mitochondrial DNA (mtDNA), cytochrome B (Cty B), and NADH dehydrogenase 5 (NADH5) contents were measured. Furthermore, hyperactivated motility (HA) and acrosome reaction rates were detected after the stimulation by progesterone with or without Quercetin, respectively. Results Quercetin could significantly improve sperm motility from the leukocytospermic patients. The level of H2O2 was significantly decreased in the supernatant of leukocytospermic patients after Quercetin treatment. The content of mtDNA in sperm was significantly decreased, whereas the contents of Cyt B and NADH 5 in sperm were significantly increased. Sperm hyperactivated motility and acrosome reaction induced by progesterone were enhanced by Quercetin in sperm from the infertile men with leukocytospermia. Conclusion These data indicate Quercetin could display protective effects against oxidative damage on sperm from the infertile men with leukocytospermia.
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