Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 11, Issue 25, Pages 22085-22095Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.9b01356
Keywords
p(HEMA-ran-GMA) nanoparticles; functionalization; protein corona; blood-brain barrier; oligodendrocyte precursor cells
Funding
- National Health and Medical Research Council (NHMRC), Australia [APP1082403]
- NHMRC Career Development Fellowship [APP1087114]
Ask authors/readers for more resources
The adsorption of serum proteins on the surface of nanoparticles (NPs) delivered into a biological environment has been known to alter NP surface properties and consequently their targeting efficiency. In this paper, we use random copolymer (p(HEMA-ran-GMA))-based NPs synthesized using 2-hydroxyethyl methacrylate (HEMA) and glycidyl methacrylate (GMA). We show that serum proteins bind to the NP and that functionalization with antibodies and peptides designed to facilitate NP passage across the blood-brain barrier (BBB) to bind specific cell types is ineffective. In particular, we use systematic in vitro and in vivo analyses to demonstrate that p(HEMA-ran-GMA) NPs functionalized with HIV-1 trans-activating transcriptor peptide (known to cross the BBB) and alpha neural/glial antigen 2 (NG2) (known for targeting oligodendrocyte precursor cells (OPCs)), individually and in combination, do not specifically target OPCs and are unable to cross the BBB, likely due to the serum protein binding to the NPs.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available