Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 11, Issue 27, Pages 23901-23908Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.9b06535
Keywords
Fe-Au barcode nanowire; single-cell immunoassay; T cell capture; interferon-gamma monitoring; tuberculosis
Funding
- National Research Foundation of Korea [2015R1A2A1A15053002, 2014M3A7B4052192, NRF-2015H1A2A1034498]
- Ministry of Science, ICT and Future Planning
- Creative-Pioneering Researchers Program through Seoul National University
- Ministry of Education
- National Research Foundation of Korea [2015H1A2A1034498, 2015R1A2A1A15053002] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Barcode nanowires (BNWs) composed of multiple layered segments of different materials are attractive to bioengineering field due to their characteristics that allow the adjustment of physicochemical properties and conjugation with two or more types of biomolecules to facilitate multiple tasks. Here, we report a metallic Fe (iron)-Au (gold) BNW-based platform for capturing CD8 T cells and the interferon-gamma (gamma) they secrete, both of which play key roles in controlling infectious diseases such as tuberculosis, at the single-cell level. We also describe an efficient approach for conjugating distinct antibodies, which recognize different epitopes to appropriate materials. The platform achieved detection even with 4.45-35.6 mu g mL(-1) of BNWs. The T cell capture efficiency was close to 100% and the detection limit for interferon-gamma was 460 pg mL(-1). This work presents a potential guideline for the design of single-cell immunoassay platforms for eliminating diagnostic errors by unambiguously identifying disease-relevant immune mediators.
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