Journal
BIOMASS CONVERSION AND BIOREFINERY
Volume 10, Issue 2, Pages 383-391Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s13399-019-00420-6
Keywords
Bacillus velezensis; Microbial lipids; Biosurfactant; Co-production; Waste office paper
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Funding
- Research Council (TRC), Oman [ORG/EBR/14/003]
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Microbial lipids and biosurfactant were successfully co-produced using waste office paper (WOP) as a substrate using a novel strain Bacillus velezensis ASN1. WOP was pretreated with 1% (v/v) H2SO4 and enzymatically hydrolyzed using cellulase (37 FPU/g solid) and beta -glucosidase (25 CBU/g solid). The total sugar yield was 21.28 g/L at 120 h and the percentage of hydrolysis was 89.07%. The obtained WOP hydrolysate was used as a substrate for the co-production of microbial lipids and biosurfactant. The best nitrogen source and carbon to nitrogen ratio for lipid production was found to be ammonium chloride and C/N 20, respectively. A lipid content of 31% was obtained with a fatty acid profile of myristic, pentadecanoic, palmitic, and stearic acid methyl esters. The crude biosurfactant was extracted by acid precipitation of the supernatant yielded 0.818 g/L and was characterized using NMR, FTIR, and LC-MS, and proved as lipopeptides. The biorefinery approach of co-producing of microbial lipid and biosurfactant using waste paper would reduce the production cost of biodiesel.
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