Journal
FOOD SCIENCE & NUTRITION
Volume 7, Issue 5, Pages 1615-1622Publisher
WILEY
DOI: 10.1002/fsn3.991
Keywords
antibody; Hg(II); immunoassay; lake water; tap water
Categories
Funding
- Jiangsu Students innovation and entrepreneurship training program project [201810289100H]
- National Key Research and development Plan [2017YFD0401404]
- National Natural Science Foundation of China [31601547]
- Fundamental Research Funds for the Provincial Universities [16KJB550003]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- Postgraduate Research and Practice Innovation Program of Jiangsu Province [KYCX17-1218, KYCX17-1219]
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In this paper, an indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) has been developed and optimized to detect Hg(II) in tap water and lake water based on a monoclonal antibody (mAb-A24). Some stabilizing additives (Gelatin, bovine serum albumin [BSA], polyvinyl alcohol [PVA], and polyvinyl pyrrolidone [PVP]) and surfactant (Tween-20) have been investigated thoroughly in the optimization process. Under the optimal condition, the 50% half maximal inhibitory concentration (IC50) and limit of detection (LOD) were 1.68 and 0.079 ng/ml, respectively. These anti-Hg mAbs also have some affinity with methyl mercury (CH3Hg) and with no cross-reactivity with other thirteen metal ions. The developed method has shown satisfactory recovery of Hg(II), ranged between 91% and 116%, from tap water and lake water. Therefore, this immunoassay can be used to detect trace Hg(II) in environment water.
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