Journal
OPEN FORUM INFECTIOUS DISEASES
Volume 6, Issue 7, Pages -Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/ofid/ofz254
Keywords
falciparum malaria; growth inhibitory activity; humoral immunity; invasion; vaccine development
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Funding
- Royal Society [AA110050]
- World Bank African Centres of Excellence Grant [ACE02-WACCBIP]
- Wellcome Trust DELTAS/African Academy of Science Grants [107755/Z/15/Z, DEL-15-007]
- National Institutes of Health/National Institute of Allergy and Infectious Diseases Grant [R01AI102848]
- Wellcome Trust [206194]
- Wellcome Trust [107755/Z/15/Z] Funding Source: Wellcome Trust
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Background. Plasmodium falciparum uses a repertoire of merozoite-stage proteins for invasion of erythrocytes. Antibodies against some of these proteins halt the replication cycle of the parasite by preventing erythrocyte invasion and are implicated as contributors to protective immunity against malaria. Methods. We assayed antibody reactivity against a panel of 9 recombinant antigens based on erythrocyte-binding antigen (EBA) and reticulocyte-like homolog (Rh) proteins in plasma from children with malaria and healthy adults residing in 3 endemic areas in Ghana using enzyme-linked immunosorbent assay. Purified immunoglobulin (Ig)G from adult plasma samples was also tested for invasion inhibition against 7 different P falciparum culture lines, including clinical isolates. Results. Antibodies against the antigens increased in an age-dependent manner in children. Breadth of reactivity to the different antigens was strongly associated with in vitro parasite growth inhibitory activity of IgG purified from the adults. The strongest predictors of breadth of antibody reactivity were age and transmission intensity, and a combination of reactivities to Rh2, Rh4, and Rh5 correlated strongly with invasion inhibition. Conclusions. Growth inhibitory activity was significantly associated with breadth of antibody reactivity to merozoite antigens, encouraging the prospect of a multicomponent blood-stage vaccine.
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