Journal
CANCER MANAGEMENT AND RESEARCH
Volume 11, Issue -, Pages 4825-4837Publisher
DOVE MEDICAL PRESS LTD
DOI: 10.2147/CMAR.S199257
Keywords
miR-193a-3p; CCND1; PDAC; proliferation
Categories
Funding
- National Natural Science Foundation of China [NSFC81560448, NSFC81560489]
- Natural Science Foundation of Guangxi, China [2014GXNSFBA118167, 2016GXNSFBA380039, 2018GXNSFAA050051]
- Guangxi Medical University Training Program for Distinguished Young Scholars (2017)
- Promoting Project of Basic Capacity for University Young and Middle-aged Teachers in Guangxi [KY2016LX031]
- Creative Research Development Grant from the First Affiliated Hospital of Guangxi Medical University
- Guangxi Zhuang Autonomous Region Health and Family Planning Commission [Z20180979]
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Background: MicroRNAs (miRNAs) could modulate gene expression at the posttranscriptional level by promoting mRNA degradation or blocking mRNA translation, thus affecting the occurrence and development of cancer. Methods: In this work, qRT-PCR was conducted to detect the expression of miR-193a-3p and CCND1. The ability of cell proliferation was evaluated via CCK-8 assay. Cell apoptosis and cell cycle distribution were detected by flow cytometry. Bioinformatic techniques were employed to research the regulatory relationship between miR-193a-3p and target genes. The relationship between miR-193a-3p and CCND1 was verified via dual-luciferase reporter assays. Results: MiR-193a-3p expression in pancreatic ductal adenocarcinoma (PDAC) tissue was significantly lower than in non-cancerous tissue. After overexpressing miR-193a-3p in PDAC cells, their multiplication ability was significantly inhibited, apoptosis was accelerated, and the cell cycle was blocked in the G1 and G2/M phases. CCND1 was confirmed to have a targeted relationship with miR-193a-3p. Moreover, CCND1 expression was significantly lower in PDAC cells with an overexpression of miR-193a-3p. Conclusions: MiR-193a-3p targeted CCND1 to suppress tumor growth in PDAC cells. MiR-193a-3p may function as a tumor inhibitor in PDAC development, which could offer a promising therapeutic and prognostic strategy for PDAC treatment.
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