Journal
ONCOIMMUNOLOGY
Volume 8, Issue 8, Pages -Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/2162402X.2019.1599635
Keywords
T cell; cytotoxicity; immunotherapy; immuno-oncology
Categories
Funding
- Cancer Research UK [A22257] Funding Source: Medline
- Wellcome Trust [WT107609] Funding Source: Medline
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In vitro assays that evaluate CD8(+) T cell-mediated cytotoxicity are important to aid in the development of novel therapeutic approaches to enhance anti-tumor immune responses. Here, we describe a novel cytotoxicity co-culture assay that circumvents the problem of highly variable allogeneic responses and obviates the constraints of HLA-restriction between effector and target cells. We show that this assay can be easily applied to a panel of tumor cell lines to provide additional insights into intrinsic drivers of sensitivity/resistance to T cell-mediated killing, and to evaluate the impact of targeted therapies on both tumor and T cell compartments.
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