Journal
MICROBIOME
Volume 7, Issue -, Pages -Publisher
BMC
DOI: 10.1186/s40168-019-0685-7
Keywords
Lipoperoxidation; Barrier function; Metabolites; Dysbiosis; Meat
Categories
Funding
- French National Agency of Research [ANR-10-ALIA-14]
- French National Institute for Agricultural Research (INRA)
Ask authors/readers for more resources
BackgroundThe World Health Organization classified processed and red meat consumption as carcinogenic and probably carcinogenic, respectively, to humans. Haem iron from meat plays a role in the promotion of colorectal cancer in rodent models, in association with enhanced luminal lipoperoxidation and subsequent formation of aldehydes. Here, we investigated the short-term effects of this haem-induced lipoperoxidation on mucosal and luminal gut homeostasis including microbiome in F344 male rats fed with a haem-enriched diet (1.5mol/g) 14-21days.ResultsChanges in permeability, inflammation, and genotoxicity observed in the mucosal colonic barrier correlated with luminal haem and lipoperoxidation markers. Trapping of luminal haem-induced aldehydes normalised cellular genotoxicity, permeability, and ROS formation on a colon epithelial cell line. Addition of calcium carbonate (2%) to the haem-enriched diet allowed the luminal haem to be trapped in vivo and counteracted these haem-induced physiological traits. Similar covariations of faecal metabolites and bacterial taxa according to haem-induced lipoperoxidation were identified.ConclusionsThis integrated approach provides an overview of haem-induced modulations of the main actors in the colonic barrier. All alterations were closely linked to haem-induced lipoperoxidation, which is associated with red meat-induced colorectal cancer risk.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available