Journal
IUCRJ
Volume 6, Issue -, Pages 412-425Publisher
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S205225251900263X
Keywords
third-generation synchrotrons; pink-beam serial crystallography; injector-based serial crystallography; structure determination; membrane proteins; protein structures; X-ray crystallography; structural biology
Funding
- Centre for Applied Structural Discovery at the Biodesign Institute at Arizona State University
- Mayo Clinic ASU Collaborative Seed Grant Award
- Flinn Foundation Seed Grant
- STC Program of the National Science Foundation through BioXFEL [1231306]
- National Science Foundation BIO ABI grant [1565180]
- National Institutes of Health (NIH) [R21DA042298, R01GM124152, R01GM095583, R35GM127086]
- National Institute of General Medical Sciences of the National Institutes of Health [R24GM111072]
- National Cancer Institute [ACB-12002]
- National Institute of General Medical Sciences [AGM12006]
- DOE Office of Science [DE-AC02-06CH11357]
- Div Of Biological Infrastructure
- Direct For Biological Sciences [1565180] Funding Source: National Science Foundation
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Since the first successful serial crystallography (SX) experiment at a synchrotron radiation source, the popularity of this approach has continued to grow showing that third-generation synchrotrons can be viable alternatives to scarce X-ray free-electron laser sources. Synchrotron radiation flux may be increased similar to 100 times by a moderate increase in the bandwidth (pink beam' conditions) at some cost to data analysis complexity. Here, we report the first high-viscosity injector-based pink-beam SX experiments. The structures of proteinase K (PK) and A(2A) adenosine receptor (A(2A)AR) were determined to resolutions of 1.8 and 4.2 angstrom using 4 and 24 consecutive 100 ps X-ray pulse exposures, respectively. Strong PK data were processed using existing Laue approaches, while weaker A(2A)AR data required an alternative data-processing strategy. This demonstration of the feasibility presents new opportunities for time-resolved experiments with microcrystals to study structural changes in real time at pink-beam synchrotron beamlines worldwide.
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