Journal
INFLAMMOPHARMACOLOGY
Volume 27, Issue 6, Pages 1297-1307Publisher
SPRINGER BASEL AG
DOI: 10.1007/s10787-019-00599-0
Keywords
Tetrastigma hemsleyanum; Concanavalin A-induced hepatitis; Th17 cells; Treg cells; Inflammatory immune response
Categories
Funding
- Natural Science Foundation of Ningbo City [2018A610430]
- New Shoot Talents Program of Zhejiang Province [2017R435001]
- Science Foundation of Zhejiang education department [Y201534579]
- Science and technology projects of Zhejiang Food and Drug Administration [YP201603]
- Leading and top talent training project of Ningbo City [NBLJ201801011]
- Key Laboratory of Ningbo, China [2016b22002]
Ask authors/readers for more resources
Objective Tetrastigma hemsleyanum, a rare and endangered medicinal plant, has attracted much attention due to its immunoregulatory and hepatoprotective activities. This study aimed to evaluate the anti-inflammatory effects and underlying mechanisms of total flavonoids from T. hemsleyanum(TFT)on Con A-induced hepatitis in mice. Methods TFT (1, 2 and 4 g/kg) and a positive control drug bifendate (200 mg/kg) were administered intragastrically to mice once daily for 10 consecutive days. On the 10th day, the model autoimmune of hepatitis was established by intravenous injection of Con A (20 mg/kg) 1 h after drug administration. Liver injury was assessed by serum levels of alanine amino transferase (ALT and AST) and histopathology 8 h after Con A injection. The levels of pro-inflammatory Th17 cytokines (IL-17, IL-6) and anti-inflammatory Treg cytokines (IL-10, TGF-beta 1) in serum were evaluated by ELISA, the levels of Th17 and Treg cells infiltrated into spleen were investigated by flow cytometry methods, and hepatic tissue transcription factor Foxp3 and ROR gamma t mRNA were determined using quantitative real-time PCR. Results Pretreatment with TFT and bifendate significantly reduced the serum levels of ALT and AST, and attenuated histopathological alterations in Con A-induced liver injury. With respect to samples treated with Con A alone, TFT and bifendate pretreatments differentially attenuated the increase of serum inflammatory factors interleukin (IL)-17 and IL-6 levels, the proportions of Th17 cells in spleen and the expression of ROR gamma t in hepatic tissues. Meanwhile, TFT and bifendate pretreatments could enhance the percentage of Treg cells in spleen and the expression of Foxp3 in hepatic tissues, as well as the levels of transforming growth factor (TGF)-beta 1, IL-10 in serum. Conclusion The anti-inflammatory effects of TFT was mediated by regulating Treg/Th17 immune homeostasis, which, therefore, suppressed the inflammatory immune response. This study provided scientific basis for the further researches and clinical applications of Tetrastigma hemsleyanum.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available