Journal
JOURNAL OF IMMUNOLOGY RESEARCH
Volume 2019, Issue -, Pages -Publisher
HINDAWI LTD
DOI: 10.1155/2019/8505021
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Funding
- Beijing Shijitan Hospital, Capital Medical University [2017-QB03]
- Beijing Key Laboratory of Cancer Therapeutic Vaccine [2017-KF01]
- Beijing Municipal Science and Technology Commission [Z181100001718090]
- Ministry of Railways [J2017Z604]
- Beijing Municipal Administration of Hospitals [PX2018029]
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Objective. This study is aimed at investigating the association of exhausted CD8(+) tumor-infiltrating lymphocytes with clinic-pathological factors. Methods. 133 patients diagnosed with primary invasive ductal breast cancer were recruited into the cross-sectional study consecutively. Immunohistochemistry was used to detect biomarker expression on formalin-fixed and paraffin-embedded sections. Double staining of CD8 and PD-1 was conducted on lymphocytes. Results. The proportion of CD8(+)/PD-1(-) TILs was 16% among patients with axillary lymph node metastasis, significantly lower than those without metastasis (24%). The expression of CK7, CK20, or Ki-67 was not related with the proportion of phenotypes of CD8/PD-1 TILs. Younger patients had more cell counts of CD8(+)/PD-1(-) TILs than elderly patients (18/HPF vs. 9/HPF, p<0.05). Patients with axillary lymph node metastasis had less CD8(+)/PD-1(-) TILs than those without metastasis (11/HPF vs. 27/HPF, p<0.05). Median counts of CD8(+)/PD-1(-) TILs among patients with CK20 and E-Cad expression were 33/HPF and 14/HPF, significantly higher than those among patients with negative CK20 (16/HPF) and E-Cad expression (6/HPF). Ki-67 index had a significant correlation with cell counts of CD8(+)/PD-1(+) TILs and CD8(+)/PD-1(-) TILs, and the correlation coefficients were 0.19 and 0.21 (p<0.05), respectively. Conclusion. The proportion of CD8(+)/PD-1(-) TILs was related with metastatic status of the axillary lymph node but cell counts of CD8(+)/PD-1(-) TILs were related with metastatic status of the axillary lymph node and expression of CK7, CK20, E-Cad, and Ki-67. Absolute cell counts, not proportion of CD8/PD-1 TILs, were more likely to distinguish clinic and pathologic characteristics of breast cancer.
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