Journal
FRONTIERS IN NEUROSCIENCE
Volume 13, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fnins.2019.00351
Keywords
human induced pluripotent stem cells; 3D-neural model system; neuronal networks; microelectrode array; synchronous activity
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Funding
- German Ministry of Education and Research [BMBF: FKZ 031B0010B]
- European Union (EuroTransBio9 project In-HEALTH)
- Alzheimerfonden [AF-556051/AF-744871, AF-640391]
- Ake Wiberg Foundation [M17-0265]
- Frederik and Ingrid Thuring Foundation [2016-0225]
- Stiftelsen Psykiatriska Forskningsfonden
- Ahlens stiftelse
- Swedish Research Council [2016-00986]
- Swedish State Support for Clinical Research [ALFGBG-427611]
- Swedish Research Council [2016-00986] Funding Source: Swedish Research Council
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Reproducibly generating human induced pluripotent stem cell-based functional neuronal circuits, solely obtained from single individuals, poses particular challenges to achieve personalized and patient specific functional neuronal in vitro models. A hallmark of functional neuronal assemblies, synchronous neuronal activity, can be non-invasively studied by microelectrode array (MEA) technology, reliably capturing physiological and pathophysiological aspects of human brain function. In our here presented manuscript, we demonstrate a procedure to generate 3D neural aggregates comprising astrocytes, oligodendroglial cells, and neurons obtained from the same human tissue sample. Moreover, we demonstrate the robust ability of those neurons to create a highly synchronously active neuronal network within 3 weeks in vitro, without additionally applied astrocytes. The fusion of MEA-technology with functional neuronal circuits solely obtained from one individual's cells represent isogenic person-specific human neuronal sensor chips that pave the way for specific personalized in vitro neuronal networks as well as neurological and neuropsychiatric disease modeling.
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