Journal
APOPTOSIS
Volume 21, Issue 11, Pages 1315-1326Publisher
SPRINGER
DOI: 10.1007/s10495-016-1294-6
Keywords
Celastrol; Angiotensin II; Mitochondrial function; NADPH oxidase; Angiotensin II type 2 receptor; Apoptosis
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Funding
- Project of Shandong Province Higher Educational Science and Technology Program [J10LF89]
- Project of Shandong Province Medicine & Health Science and Technology Program [2011HZ003]
- Natural Science Foundation of Shandong Province [ZR2012HM076]
- Foundation of Taishan Scholar [tshw20110515]
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Angiotensin II (AngII) is an important factor that promotes the proliferation of cancer cells, whereas celastrol exhibits a significant antitumor activity in various cancer models. Whether celastrol can effectively suppress AngII mediated cell proliferation remains unknown. In this study, we studied the effect of celastrol on AngII-induced HepG2 cell proliferation and evaluated its underlying mechanism. The results revealed that AngII was able to significantly promote HepG2 cell proliferation via up-regulating AngII type 1 (AT(1)) receptor expression, improving mitochondrial respiratory function, enhancing nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity, increasing the levels of reactive oxygen species (ROS) and pro-inflammatory cytokines. The excess ROS from mitochondrial dysfunction is able to cause the apoptosis of tumor cells via activating caspase3 signal pathway. In addition, the reaction between NO and ROS results in the formation of peroxynitrite (ONOO-), and then promoting cell damage. celastrol dramatically enhanced ROS generation, thereby causing cell apoptosis through inhibiting mitochodrial respiratory function and boosting the expression levels of AngII type 2 (AT(2)) receptor without influencing NADPH oxidase activity. PD123319 as a special inhibitor of AT(2)R was able to effectively decreased the levels of inflammatory cytokines and endothelial nitric oxide synthase (eNOS) activity, but only partially attenuate the effect of celastrol on AnII mediated HepG2 cell proliferation. Thus, celastrol has the potential for use in liver cancer therapy. ROS derived from mitochondrial is an important factor for celastrol to suppress HepG2 cell proliferation.
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