Journal
FRONTIERS IN MICROBIOLOGY
Volume 10, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2019.01077
Keywords
influenza A virus; nasal swab sample; wash-free; handheld; magnetic bioassay; giant magnetoresistance; on-site diagnosis
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Funding
- National Science Foundation MRSEC facility program
- Distinguished McKnight University Professorship
- Centennial Chair Professorship
- Robert Hartmann Chair Professorship
- UROP program from the University of Minnesota
- USDA-NIFA, SAES Funds [MIN-62-097]
- Institute of Engineering in Medicine of the University of Minnesota, College of Veterinary Medicine Emerging, Zoonotic and Infectious Diseases Signature Program
- MNDrive: OVPR STEMMA program
- NSF
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The dissemination of Influenza A virus (IAV) throughout the world has become one of the main concerns for the health of both animals and human beings. An efficient and sensitive diagnostic tool is thus needed for the early detection of IAV. Here, we developed a wash-free magnetic bioassay and further integrated it with a handheld platform based on giant-magnetoresistance (GMR) sensors. The wash-free magnetic bioassay significantly accelerates and simplifies the detection process. This brand-new system was successful in detecting both IAV nucleoprotein and IAV-contained nasal swab samples from pigs on the farm. The limit of detection (LOD) is 0.3 nM for IAV nucleoprotein and 250 TCID50/mL for IAV-spiked nasal swab samples. The detection of nasal swab samples containing unpurified IAV was also performed, demonstrating the capability of the magnetic wash-free assay in the detection of biomarkers in complex sample matrix.
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